WebFITC ( fluorescein isothiocyanate) is widely used to label proteins, antibodies, peptides, hormones, amine-modified oligonucleotides, and other amine-containing molecules with … WebThe FITC solution is then diluted (1:50, 1.5:100, 1:100, 1:200) with PBS buffer by the students. 2.2. Bioconjugation. The protocol used for the modification of BSA with FITC is reported in literature 1 and is very simple: 1) Students are asked to prepare a 2 mg/ml BSA solution in 0.1 M sodium carbonate, pH 9.

FITC labeling & conjugation protocols & theory - Papyrus Bio Blog

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FITC-conjugated cyclic RGD peptides as fluorescent probes for …

WebFAM (Carboxyfluorescein) is a fluorescent compound with an excitation peak at 493 nm and an emission peak at 517 nm. It can be excited using a 488 nm laser paired with a 530/30 … WebFeb 10, 2024 · Py 3-FITC can be used to detect TGF-β1-induced collagen type I. HFL-1 cells were treated with TGF-β1 for 0 h (a–c) or 48 h (d–f) and then stained with Py 3-FITC (a,d) and collagen type I antibody (b,e), and their merged images are shown (c,f). Collagen type I expression was increased by 48 h of TGF-β1 treatment. WebDual-modal imaging contrast agents with unique X-ray computed tomography (CT) and optical imaging capabilities have attracted much attention in recent years. Herein, the new silica hybrid nanocomposites containing Au nanoparticles and FITC dyes (FITC–Au@SiO2) were investigated as this kind of contrast agent. TEM ch razer light projector