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WebSocial media has become an inescapable and intrinsic part of our lives. It has profound implications on our politics, society and so much more. It is also br... Before testing the hypothesis that active CDCs are cleared by microvesicular shedding, we first needed to determine the sublytic dose for each CDC and if it was equivalent between cell types and CDCs. We compared SLO with ILY, which requires human CD59 for cytotoxicity,3 and PFO. In order to compare … See more Cell-specific sensitivity may be due to differential CDC binding. To test this hypothesis, we used SLO, which showed similar toxicity across … See more Although toxin binding was not related to SLO resistance, MV shedding has been related to SLO resistance in HeLa, HEK, and CHO cells.24, 34 We tested if shedding was a general mechanism of CDC resistance. We … See more As MV shedding was only triggered by active toxins, it is possible that active pores are shed, whereas inactive toxin is endocytosed. The methods used to assess toxin endocytosis19, 20, 21, 40 could not … See more To determine whether MV shedding is a conserved repair mechanism between cell types, we compared the cellular responses with sublytic toxin challenges (Figure 3). We observed CDC, … See more dr toth surgeon
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