Myc agarose beads
WebConfocal image of HEK293T cells co-transfected with HA- or Myc- tagged DDX1, DDX21, DHX36 or TRIF expression plasmids. Cells without stimulation ... Depletion with … Web4 aug. 1998 · GST and GST-Pam0 proteins were incubated with a nuclear extract of CB33-Myc cells, recovered on glutathione agarose beads, and analyzed by Western blotting …
Myc agarose beads
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WebPierce Anti-c-Myc Agarose consists of a highly specific monoclonal anti-c-Myc antibody that is covalently immobilized on a crosslinked 6% beaded agarose support. The product is … Web12 jan. 2024 · CUT&RUN (Cleavage Under Targets & Released Using Nuclease) outlines antibody-targeted DNA-binding proteins inches situ with high resolution and low background, providing a simple, robust and scalable alternative to chromatin immunoprecipitation.
Web29 apr. 2024 · The supernatants were treated with protein A/G plus agarose (sc-2002; Santa Cruz Biotechnology, CA, USA) for 1.0 h at 4 °C and immunoprecipitated using anti-FLAG beads. The beads were washed with NP-40 buffer and resolved using standard SDS-PAGE. For the GST pull-down assays, FLAG-PCV3-Cap or FLAG-PCV4-Cap was used … WebYou can use this antibody in combination with Protein G agarose beads from any company for the IPs. Also, if you want to directly use antibody-conjugated beads for IPs, Sigma …
WebV5 agarose beads Log in / Register ... Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in … WebM047-8 Anti-Myc-tag mAb-Agarose (PL14) ... Mix 20 L of 50% protein A agarose beads slurry resuspended in 300 L of IP buffer [10 mM Tris-HCl (pH 8.0), 500 mM NaCl, 0.1% …
WebThermo Scientific Pierce 抗 c-Myc 磁珠是用于借助手动或自动磁性分离器对细菌或哺乳动物细胞或 体外 系统中表达的重组 c-Myc 标记蛋白进行免疫沉淀的亲和微粒。. 抗 c-Myc …
WebBriefly, streptavidin agarose beads (Millipore, Burlington, MA, USA) were pre-washed once with 1× mild buffer (50 mM Tris HCl pH 7.5, 150 mM NaCl, 0.1% NP-40, 5 mM EDTA, 5 mM EGTA, 15 mM MgCl 2 ), and 15 μg of either biotinylated LFV-Z-WT or LFV-Z PPxY mutant peptide was incubated with the pre-washed streptavidin beads in 500 μL of 1× mild … ghosts of the heartWebThis product is a conjugate of activated magnetic cross-linked agarose beads to a polyclonal antibody that was raised against the c-Myc sequence. The c-Myc peptide tag … front porch urn ideasWeb1. Immunoprecipitation using Protein A/G PLUS Agarose Beads. Cell Lysate Preparation. Centrifuge 10 8 thymocytes in a microcentrifuge at 13,000 rpm for 3 min and remove the supernatant. Note: The cell number will vary depending on the expression levels of the desired protein and the chosen cell type. Re-suspend the cells in 500 µL lysis buffer … front porch urn planter ideashttp://biopioneer.com.tw/?p=41026 front porch usesWeb11 apr. 2024 · Myc-tagged versions of these mutants, along with the wild-type (WT) protein, were ectopically expressed in iKras PDAC cells ... Agencourt AMPure XP beads: Beckman Coulter: A63880: CircLigase II ssDNA ligase kit: Epicentre: ... Ph2AX antibody was initially tested on cisplatin-treated and untreated iKras PDAC cells embedded in agarose, ... ghosts of the haunted mansionWeb5. 将细胞裂解产物加入到平衡的Myc tag-Nanobody-Agarose Beads中（如果未做第4步，可在细胞裂解产物中直接加入 20ul beads悬液），在4 ℃冷柜中的旋转混合仪上结合30 … ghosts of the jem\u0027hadarWeb1 jun. 2024 · ERK1, BRD4, and MYC immunoblots of pull-down reactions with equimolar amounts of MYC (0.3 µg) immobilized on agarose beads with ERK1 (0.2 µg) alone, … front porch upholstery